Prevalence and Occurrence of Various Wilt Pathogens Associated with Tomato (Solanum lycopersicum L.) in Togo

Main Article Content

Kwasi Dzola Ayisah
Koffi Simiti
Mawuli Kossivi Aziadekey

Abstract

In December 2015, wilt symptoms on tomato plants (Solanum lycopersicum L.) were observed on vegetable growing perimeters in Sotouboua district in Togo. The disease, manifested by wilting of the youngest leaves followed by wilting and total desiccation of plants who eventually dy, leading to losses of up to 100%, is similar to bacterial wilt. The aim of this study, was to determine the pathogen responsible for the observed symptoms. For this purpose, phytosanitary surveys were carried out on tomato plots in Sotouboua distric, in 2018. During the surveys, Tomato plants infected by wilt and the plots soil samples, were collected on CECODRI project and farmers’ plots in the district. Soil samples were analyzed for the detection of nematodes while, tomato leaves, stems and roots were directly observed under binocular loupe and after incubation in Petri dishes containing filter paper moistened with distilled water to encourage sporulation of phytopathogenic fungi. Infected tomato stems and roots were analyzed by stem-streaming and DAS-ELISA tests using Agdia inc. Ralstonia solanacearum Patho Screen Kit to detect R. solanacearum. The results of the phytosanitary surveys showed that the wilt prevalence was 100% in Sotouboua district with incidence rates of up to 100%. Analysis of diseased samples, using stem-streaming and DAS-ELISA tests, revealed that 85.11% of diseased plants were infected by R. solanacearum. No nematodes were identified in the roots of the infected plants, but in soil samples only a few nematodes were counted. No fungus was found in the plants with wilt symptoms. It appears, therefore that, the wilt on tomato plants in Sotouboua district was caused by R. solanacearum. This, in our knowledge, is the first report on R. solanacearum infection on tomato in Togo.

Keywords:
Tomato, bacterial wilt, Ralstonia solanacearum.

Article Details

How to Cite
Ayisah, K. D., Simiti, K., & Aziadekey, M. K. (2019). Prevalence and Occurrence of Various Wilt Pathogens Associated with Tomato (Solanum lycopersicum L.) in Togo. Asian Journal of Agricultural and Horticultural Research, 4(4), 1-9. https://doi.org/10.9734/ajahr/2019/v4i430032
Section
Original Research Article

References

Laterrot H. Production et données économiques de la tomate. INRA; 2013.
[Accessed 27 september 2019]
Available:http://ephytia.inra.fr/fr/C/4945/Tomate-Production-et-donnees-economiques-de-la-tomate. French.

FAOSTAT FAO. Agriculture database. FAO statistical yearbook 2016. Viale delle Terme di Caracalla 00153 Rome, Italy ; 2016.

ITRA (Institut Togolais de Recherche Agronomique). Rapport annuel. Lomé-Togo. 2008;34. French.

F.A.O. Agriculture database. FAO statistical yearbook 2017. Viale delle Terme di Caracalla 00153 Rome, Italy ; 2017.

DSID (Direction de la Statistique de l’Information et de la Documentation). Enquête-Budget-Consommation (EBC) N°7-Lomé. Ministère du Développement Rural. Lomé-Togo, Lomé-Togo. 1991;74. French.

DESA (Direction des Enquêtes et Statistiques Agricoles). Résultats définitifs du récensement national des exploitants maraîchers en milieu urbain et péri-urbain – Lomé. Ministère du Développement Rural, Lomé-Togo. 1993;72. French.

Gilbertson RL, Kane M, Kane PD, Miller S. Le flétrissement bactérien de la tomate en Afrique de l’Ouest: Cause, caractéristiques et gestion. Integrated Pest Management Collaborative Research Support Program (IPM CRSP). Bureau international de recherche, éducation et développement. Virginia Tec. International Affairs Offices (IAO), 526 Prices Fork Road | Blacksburg, VA 24061, USA. 2001;2. French.

Radhi MZA, Adam MB, Saud HM, Hamid MN, Tony PSH, Tan GH. Efficacy of smart fertilizer for combating bacterial wilt disease in Solanum lycopersicum. J. Agric. Food Sci. 2016;4:137-143.

Sikirou R, Beed F, Ezin V, Hoteigni J, Miller SA. Distribution, pathological and biochemical characterization of Ralstonia solanacearum in Benin. Ann. Agri.Scie. 2017;62:83–88.

Subedi N, Gilbertson RL, Osei MK, Miller SA, Cornelius E. First Report of Bacterial Wilt Caused by Ralstonia solanacearum in Ghana, West Africa. Plant Disease, Published by The American Phytopatho-logical Society. 2014;98(6):840.

Son D, Somda I, Legreve A, Schiffers B. Pratiques phytosanitaires des producteurs de tomates du Burkina Faso et risques pour la santé et l’environnement. Cah. Agric. 2017;26:25005. French.

Bertrand C, Lizot JF, Mazollier C. Lutter contre les nématodes à galles en Agriculture Biologique. GRAB (Groupe de recherche en agriculture biologique). Site Agroparc - BP 1222 - 84911 AVIGNON Cedex 9. 200;2. French.

Naika S, De Jeude JVL, De Jeffau M, Hilmi M, Van Dam B. La culture de tomate, production, transformation et commercialisation. Ed. Wageningen, Pays bas. 2005;105. French.

Peeters N, Guidot A, Vailleau F, Valls M. Ralstonia solanacearum, a widespread bacterial plant pathogen in the post-genomicera. Mol. Plant Pathol. 2013b;14: 651–662.

Kummert J, Lepoivre P. Phytopathologie, Le diagnostic : Détection et identification des agents phytopathogènes. Les Presses Agronomiques de Gembloux, Belgique. 2007;215-230. French.

ITRA (Institut Togolais de Recherche Agronomique). Stratification du Togo en zones homogènes pour la recherche agronomique In : Rapport Annuel 2009. ITRA. Lomé, Togo. 2009;25-28. French.

Amouzou TC. Rapport d'identification de CECODRI (Centre Coopérative pour le Développement Rural Intégré), Gembloux. 2000;39. French.

Evoda MK. Evaluation de cultivars de tomate (Lycopersicon esculentum Mill) : Potentialités de culture en contre saison dans la région maritime au Togo. Mémoire présenté en vue de l’obtention du grade d’INGENIEUR AGRNOME, ESA-UL. 1988; 85. French.

FAO. Catalogue officiel des espèces et des variétés cultivées au Sénégal. République du Sénégal, Ministère de l’Agriculture et de l’Équipement Rural. 2012;212.
[Accessed. 23 october 2019]
Available:http://www.fao.org/pgrfa-gpa- archive/sen/docs/senegal_varietes/Catalogue_%20varietes.htm. French.

Nutter FW Jr, Teng PS, Shokes FM. Disease assenment terms and concepts. Plant Diseases. 1991;75:1187-1188.

EPPO (European and Mediterranean Plant Protection Organization). Ralstonia solanacearum. EPPO, Bulletin. 2004;34: 173–178.

Blancard D. Les maladies de la tomate : Identifier, Connaître, Maîtriser. Quae. 2009;679. French.

Fontem AD. Dynamics and integrated management of Potato Late Blight in Cameroon. Thèse de Doctorat ès Sciences Naturelles, Option : Biologie de Développement, Spécialité: Phytopatho-logie. Université du Bénin, Faculté des Sciences. Lomé – Togo. N°d’ordre 0029. 1998;178.

Taylor AL. Introduction à la recherche sur les nématodes phytoparasites. Manuel FAO pour l’étude des nématodes phytoparasites et les moyens de lutte. FAO, Rome. 1968;134. French.

Sanboga K. Les nématodes de la tomate: Identification et contrôle avec Glomus mosseae et avec des poudres de feuilles de neem et gousse de néré. Mémoire de fin d’étude agronomique. N° 07/04/PV. Université de Lomé, Ecole Supérieure d’Agronomie, Lomé-Togo. 2007;72. French.

Waiganjo MM, Wabule NM, Nyongesa D, Kibaki JM, Onyango I, Wepukhulu SB, Muthaka NM. Tomato production in Kirinyaga district Kenya, a baseline survey report. KARI/IPM CRSP, Nairobi, Kenya. 2006;1-43.

López MM, Biosca E. Potato bacterial wilt management: new prospects for an old problem. In Allen C, Prior P and Hayward AC (Ed). Bacterial Wilt Disease and the Ralstonia solanacearum Species Complex. St. Paul, Minn. American Phytopathological Society. 2005;205– 224.

Aloyce A, Ndakidemi PA, Mbega ER. Identification and Management Challenges Associated with Ralstonia solanacearum (Smith), Causal Agent of Bacterial Wilt Disease of Tomato in Sub-Saharan Africa. Pak. J. Biol. 2017;20:530-542.

Álvarez B, Biosca EG, López MM. On the life of Ralstonia solanacearum, a destructive bacterial plant pathogen. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology (ed. A. Méndez‐Vilas), Formatex, Badajoz, Spain. 2010;267–279.

Jatala P, Martin C, Mendoza HA. Role of nematodes in disease expression by Pseudomonas solanacearum and strategies for screening and breeding for combined resistance. In: Report of the planning conference on bacterial diseases of potato 1987. International Potato Centre, Lima, Peru. 1988;35–37.

Singh D, Yadav DK, Sinha S and Choudhary G. Effects of temperature, cultivars, injury of root and inoculum load of Ralstonia solanacearum to cause bacterial wilt of tomato. Phyto. Plant Prot. 2014b;47(13):1574-1583.