Age-related Induced Resistance Effect on Tomato Seedlings for Producing Tomato Yellow Leaf Curl Virus (TYLCV)-Free Plants and High-quality Seeds

H. H. Hamed *

Vegetable Seed Production Technology Research Department, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt.

A. Z. Hegazi

Vegetable Seed Production Technology Research Department, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt.

T. G. Anany

Vegetable Seed Production Technology Research Department, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt.

A. F. E. Afsah

Vegetable and Aromatic Plant Insects Department, Plant Protection Research Institute, Agricultural Research Center, Giza, Egypt.

*Author to whom correspondence should be addressed.


Abstract

Egypt is facing a major problem in the field of tomato seed production, as infection with the yellow tomato leaf curl virus (TYLCV) is one of the most important factors in the success of this important production process, which has an impact on national food security, in addition to facing the steady increase in the costs of importing tomato seeds in particular vegetable crop seeds in general. Therefore, the main objective of the current study is to study plant age-related induced resistance (ARIR) against tomato yellow leaf curl virus (TYLCV) in tomato plants. Several research points were studied, respectively: first, the effect of plant age on resistance to TYLCV virus in tomato plants that is transmitted by whitefly. Second, the detection and identification of tomato yellow leaf curl virus (TYLCV) in seeds obtained from seedlings of different ages (35 and 90 days old). Third, study the behavior of the whitefly in terms of the number of eggs and larvae, the percentage of the number of infected plants that showed symptoms of infection with the virus, and its relationship to the age of the seedlings. The results of this study proved that the age of the plant is closely related to the ability of the plant to withstand infection with the tomato yellow leaf curl virus (TYLCV). The DNA of the tomato yellow leaf curl virus (TYLCV) was identified from a sample of seeds obtained from plants obtained from 35-day-old seedlings. On the contrary, the DNA of tomato yellow leaf curl virus (TYLCV) was not detected in the seed sample obtained from plants produced from 90-day-old seedlings that were cultivated and adapted inside the nursery. The results also showed that in both protocols, using or without insecticides did not prevent the white fly from laying eggs and producing larvae on the plants. The increase was also gradual in the numbers of eggs and larvae of the white fly, as this activity peaked in the third week of transferring the seedlings to the open field, then those numbers decreased after the third week. This study also demonstrated the effect of positive seedling age (90 days old) on morphological traits related to vegetative growth, fruit production, and seed yield. Among the important benefits obtained was the ability to obtain seeds free of TYLCV in tomato plants, as well as the ability to remove nursery plants that showed early symptoms of the virus, and thus reduce the economic losses caused by the whitefly through the spread of the virus in the open fields.

Keywords: Tomato, Lycopersicon esculentum L., age-related resistance effect, ARIR, whitefly, seed production, tomato yellow leaf curl virus, TYLCV


How to Cite

Hamed , H. H., Hegazi , A. Z., Anany , T. G., & Afsah , A. F. E. (2023). Age-related Induced Resistance Effect on Tomato Seedlings for Producing Tomato Yellow Leaf Curl Virus (TYLCV)-Free Plants and High-quality Seeds. Asian Journal of Agricultural and Horticultural Research, 10(3), 28–42. https://doi.org/10.9734/ajahr/2023/v10i3229

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References

Willcox JK, Catignani GL, Lazarus S. Tomatoes and cardiovascular health. Critical Review in Food Science and Nutrition. 2003;43(1):1–18.

Bramley PM, Is lycopene beneficial to human health? Phytochemistry. 2000;54 (3):233–236.

Klipstein-Grobush K, Launer LJ, Geleijnse JM, Boeing H, Hofmann A, Witte-man JC, Serum carotenoids and atherosclerosis: the Rotterdam Study. Atherosclerosis. 2000;148:49–56.

Giovannucci E, Rimm EB, Liu Y, Stampfer MJ, Willett WC. A prospective study of tomato products, lycopene, and prostate cancer risk. Journal of National Cancer Institute. 2002;94(5):391–398.

Riso P, Visioli F, Erba D, Testolin G, Porrini M. Lycopene and vitamin C concentrations increase in plasma and lymphocytes after tomato intake. Effects on cellular antioxidant protection. European Journal of Clinical Nutrition. 2004;58(10): 1350–1358.

Krinsky NI, Johnson EJ. Carotenoids actions and their relation to health and disease. Molecular Aspects of Medicine. 2005;26 (6):459–516.

FAOSTAT. Food and Agriculture Organization Corporate Statistical Database; 2021.

Picó B, Díez MJ, Nuez F. Viral diseases cause the greatest economic losses to the tomato crop. II. The Tomato yellow leaf curl virus—A review. Scientia Horticulturae. 1996;67(3-4):151-196.‏

Kil E, Kim S, Lee Y, Byun H, Park J, Seo H, Kim C, Shim J, Lee J, Kim J, Lee K, Choi H, Lee S. Tomato yellow leaf curl virus (TYLCV-IL): a seed-transmissible geminivirus in tomatoes. Sci. Rep. 2016; 6(1). DOI: 10.1038/srep19013

Kil EJ, Park J, Choi HS, Kim CS, Lee S. Seed transmission of tomato yellow leaf curl virus in white soybean (Glycine max). The Plant Pathology Journal. 2017;33(4): 424–428. Available:https://doi.org/10.5423/PPJ.NT.02.2017.0043

Panter SN, Jones DA. Age-related resistance to plant pathogens. Adv. Bot. Res. 2002;38:251–280. DOI: 10.1016/S0065-2296(02)38032-7

Hu L, Yang L. Time to fight: molecular mechanisms of age-related resistance. Phytopathology. 2019;109(9):1500–1508. DOI: 10.5423/PPJ.RW.12.2019.0295

Moriones E, Aramburu J, Riudavets J, Arnó J, Laviña A. Effect of plant age at time of infection by tomato spotted wilt tospovirus on the yield of field-grown tomato. Eur. J. Plant Pathol. 1998;104: 295–300. DOI: 10.1023/A:1008698731052

Beaudoin ALP, Kahn ND, Kennedy GG. Bell and banana pepper exhibit mature-plant resistance to tomato spotted wilt tospovirus transmitted by Frankliniella fusca (Thysanoptera: Thripidae). J. Econ. Entomol. 2009;102(1):30–35. DOI: 10.1603/029.102.0105

Byamukama E, Robertson AE, Nutter FW. Bean pod mottle virus time of infection influences soybean yield, yield components, and quality. Plant Dis. 2015; 99(7):1026–1032. DOI: 10.1094/PDIS-11-14-1107-RE

Levy D, Lapidot M. Effect of plant age at inoculation on the expression of genetic resistance to tomato yellow leaf curl virus. Arch. Virol. 2008;153(1):171–179. DOI: 10.1007/s00705-007-1086-y

Pastor-Fernández J, Sánchez-Bel P, Flors V, Cerezo M, Pastor V. Small signals lead to big changes: the potential of peptide-induced resistance in plants. J. Fungi. 2023;9:265. Available:https://doi.org/10.3390/jof9020265

Reglinski T, Havis N, Rees H, de Jong H. 2023 The practical role of induced resistance for crop protection. Phytopathology. Epub ahead of print. PMID: 36636755. DOI: 10.1094/PHYTO-10-22-0400-IA

Oriani MADG, Vendramim JD. Influence of trichomes on attractiveness and ovipositional preference of Bemisia tabaci (Genn.) B biotype (Hemiptera: Aleyrodidae) on tomato genotypes. Neotropical Entomology. 2010;39(6):1002–1007. Available:https://doi.org/10.1590/s1519-566x2010000600024

Fakorede MAB, Ojo DK. Variability for seedling vigor in maize. Exp. Agric. 1981;17(2):195-201.

Watson DJ. Leaf growth in relation to crop yield. In: The Growth of Leaves (Ed. F.L. Milthorpe) Butterworth, U.K. 1956;178–191.

Sambrook J, Russel D. Molecular cloning: A laboratory manual, 3rd Edn. Cold Spring Harbor, Ny, USA, Cold spring Harbor Laboratory Press; 2001.

Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual. Book : No. Ed. 1989;2-1546.

Gomez KA, Gomez AA. Statistical Procedures for Agricultural Research. New York: John Wiley and Sons Inc. New York. 1984;67-215.

Minitab. MINITAB 16. MINITAB User’s guide. Minitab Inc, Harrisburg, Pennsylvania USA; 2010.

Lapidot M, Friedmann M. Breeding for resistance to whitefly-transmitted geminiviruses. Ann. Appl. Biol. 2002;140 (2):109-127.

Eigenbrode SD, Espelie KE. Effects of plant epicuticular lipids on insect herbivores. Annu Rev Entomol. 1995;40 (1):171–194.

Broekgaarden C, Riviere P, Steenhuis G, Del sol Cuenca M, Kos M, Vosman B. Phloem-specific resistance in Brassica oleracea against the whitefly Aleyrodes proletella. Entomol Exp Appl. 2012; 142(2):153–164. Available:https://doi.org/10.1111/j.1570-7458.2011.01210.x

Leite GLD, Picanço M, Guedes RNC, Zanuncio JC. Role of plant age in the resistance of Lycopersicon hirsutum f. glabrate to the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae). Sci Hortic. 2001;89(2):103–113 Available:https://doi.org/10.1016/S0304-4238(00)00224-7

De Kogel WJ, Balkema-Boomstra A, Van der Hoek M, Zijlstra S, Mollema C. Resistance to western flower thrips in greenhouse cucumber: effect of leaf position and plant age on thrips reproduction. Euphytica. 1997;94(1): 63–67. Available:https://doi.org/10.1023/A:1002937709157

Leiss KA, Choi YH, Abdel-Farid IB, Verpoorte R, Klinkhamer PGL. NMR Metabolomics of thrips (Frankliniella occidentalis) resistance in Senecio hybrids. J Chem Ecol. 2009;35(2):219–229.

Available:https://doi.org/10.1007/s10886-008-9586-0

Osterbaan LJ, Fuchs M. Dynamic interactions between plant viruses and their hosts for symptom development. J. Plant Pathol. 2019;101(4):885–895. DOI:10.1007/s42161-019-00323-5

Velásquez AC, Castroverde CDM, He SY. Plant-pathogen warfare under changing climate conditions. Curr. Biol. 2018;28 (10):619–634. DOI: 10.1016/j.cub.2018.03.054