Impact of Foliar Spraying by Using Some Stimulative Substances on Growth, Green Pods, Dry Seed Yield, Its Components and Some Chemical Constituents of Pea Plants (Pisum sativum L.) under High Temperature Stress Conditions

Ashraf Y. Ismail *

Vegetable Research Department, Horticulture Research Institute, Agricultural Research Center (A.R.C.), Giza, Egypt.

Medhat A. Abdel-Aziz

Vegetable Research Department, Horticulture Research Institute, Agricultural Research Center (A.R.C.), Giza, Egypt.

*Author to whom correspondence should be addressed.


This experiment was carried out during the two winter seasons of 2020/2021 and 2021/2022 at Qaha Vegetable Research Farm (Qalubia Governorate), Horticulture Research Institute, Agriculture Research Center (A. R. C.), Egypt. The objectives of this study were to investigate the effect of foliar spraying with some stimulative substances i.e. Green Miracle at the rate of 3 cm3 / L, Kaolin (Aluminum silicate) at the rate of 20 g/ L, Selenium element at the rate of 12 mg /L, Megacal Hort at the rate of 2.5 cm3/L, Super Grow Hortic at the rate of 0.75 cm3 /L and High Harvest at the rate of 0.75 cm3/L beside the control treatment (spraying with tap water) on vegetative growth, green pods, dry seed yield and its components as well as seed quality of pea Master B cv. under high temperature stress conditions. The experimental design was a randomized complete block containing seven treatments with three replicates. Seeds of peas were sown in the 2nd week of September during the two winter growing seasons.

The results showed that, the most estimated parameters were significantly affected with all tested treatments as foliar applications; the superiority effect of the treatments was recorded by using kaolin (Aluminum silicate) followed by selenium element and green miracle which led to obtain the highest significant increase in all the previous studied traits i.e. the vegetative growth, green pods yield, dry seed yield and its components as well as seed quality under high temperature stress conditions. The superior treatment; kaolin at the rate of 20 g/ L increased green pod yield by 36.8 and 33.6 % and enhanced dry seed yield /fed by 34.2 and 32.3 % over the control treatment (the untreated plants) in the two winter seasons, respectively. 

Keywords: Peas, high temperature stress, green miracle; kaolin, selenium, Megacal Hort, super grow hortic, high harvest, dry seeds, carbohydrates and protein

How to Cite

Ismail , A. Y., & Abdel-Aziz , M. A. (2023). Impact of Foliar Spraying by Using Some Stimulative Substances on Growth, Green Pods, Dry Seed Yield, Its Components and Some Chemical Constituents of Pea Plants (Pisum sativum L.) under High Temperature Stress Conditions. Asian Journal of Agricultural and Horticultural Research, 10(4), 110–130.


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Fikreselassie M. Variability, heritability and association of some morpho-agronomic traits in field pea (Pisum sativum L.) genotypes. Pakistan J. of Bio. Sci. 2012;15(8):358.

Lamichaney A, Parihar AK, Hazra KK, Dixit GP, Katiyar PK, Singh D. Untangling the influence of heat stress on crop phenology, seed set, seed weight and germination in field pea (Pisum sativum L.). Front. Plant Sci. 2021;12.

DOI: 10.3389/ fpls, 635868.

Medina E, Kim SH, Yun M, Choi WG. Recapitulation of the function and role of ROS generated in response to heat stress in plants. Plants. 2021;10:371. DOI: 10.3390/plants10020371.

Devi J, Sagar V, Mishra GP, Jha PK, Gupta N, Rakesh K, Dubey K, Singh PM, Behera TK, Prasad PVV. Heat stress tolerance in peas (Pisum sativum L.): Current status and way forward. Front. Plant Sci. 2023;13: 1-19.

Dixon RA. Natural products and plant disease resistance. Nature. 2001;411: 843–847.

Szabados L, Savoure A. Proline: A multifunctional amino acid. Trends Plant Sci. 2010;15:89–97.

Gong HJ, Chen KM. The regulatory role of silicon on water relations, photosynthetic gas exchange and carboxylation activities of wheat leaves in field under drought conditions. Act. Physiol. Plant. 2012; 34:1589 – 1594.

Kim YH, Khan AL, Waqas M, Lee IJ. Silicon regulates antioxidant activities of crop plants under abiotic-induced oxidative stress: a review. Front Plant Sci. 2017;8:510.

Koteswara RG, Babu MS, Sravani V, Sindhuja M. A review on-influence of antitranspirants (ATS) in vegetable crops. Int. J. Pure App. Biosci. SPI. 2018;6(3):394-399.

Ahmed A. Impacts of kaolin and pinoline foliar application on growth, yield and water use efficiency of tomato (Solanum lycopersicum L.) grown under water deficit. J. Saudi Soc. Agric. Sci. 2019;18:256–268.

Ahmed HMI, Dawa KK, Abd El-Nabi HME, Makeen A. Influence of some irrigation levels and foliar application of some antitranspirants on vegetative growth, leaf chemical constitutes and seed yield of squash plants. J. Plant Production, Mansoura Univ. 2019; 7:505 – 510.

Ismail AY, Fayed AAM. Response of dry seeds yield of faba bean (Vicia Faba, L.) to spraying with amino acids, organic acids, (NAA) growth regulator and micro nutrients. Alex. J. Agric. Sci. 2020;1:7–16.

Tapiero H, Townsend DM, Tew KD. The antioxidant role of selenium and selenocompounds. Biomed. Pharmacother. 2003;57(3- 4):134-144.

Shang QM, Shu CF, Zhi ZG. Regulation of selenium on antioxidative enzymes activity in pepper leaves under high temperature stress. Acta Hort. Sinica. Chinese Society for Hort. Sci., Beijing, China. 2005;32:35-38.

Feng RC, Wei, Tu S. The roles of selenium in protecting plants against abiotic stresses. Environ. Exp. Bot. 2013;87:58–68.

Wathiq M, Hmood MER, Sabry MY, Amr AM. Effect of selenium and silicon foliar applications on the growth and yield of common bean. Egyptian J. of Hort. 2022;2:147-158.

Zhu Z, Gerendas J, Bendixen R, Schinner K, Tabrizi H, Sattelmacher B, Hansen UP. Different tolerance to light stress in N03–and NH4+- grown Phaseolus vulgaris L. Plant Biol. 2000;2:558–570.

Banerjee P, Visha K, Nath R, Bandyopadhyay P. Seed priming and foliar nutrition studies on relay grass pea after winter rice in lower Gangetic plain. J. Crop. Weed. 2019;15:72–78.

Barker AV, Pilbeam DJ. Handbook of Plant Nutrition, CRC Press New York; 2006.

Waraich EA, Ahmad R, Ashraf MY, Saifullah AM. Improving agricultural water use efficiency by nutrient management in crop plants. Acta Agric., Scandinavica, Section B - Plant Soil Sci. 2011;61(4):291-304.

Hasanuzzaman M, Hossain MA, Silva JATD, Fujita M. Plant responses and tolerance to abiotic oxidative stress: Antioxidant defiance is a key factor. In Crop Stress and Its Management: Perspectives and Strategies; Bandi V, Shanker AK, Shanker C, Mandapaka, Eds.; Springer: Berlin/Heidelberg, Germany. 2012;261–316.

Parvin K, Nahar K, Hasanuzzaman M, Bhuyan MHM, Fujita M. Calcium-mediated growth regulation and abiotic stress tolerance in plants. In plant abiotic stress tolerance; Springer: Cham, Switzerland. 2019;291–331.

Shulaev V, Cortes D, Miller G, Mittler R. Metabolomics for plant stress response. Physiol. Plant. 2008;132(2): 199-208.

Liu Y, Li J, Zhu Y, Jones A, Rose RJ, Song Y. Heat stress in legume seed setting: effects, causes and future prospects. Front. Plant Sci. 2019; 10.

DOI: 10.3389/ fpls, 00938.

Abeysingha G. The effects of auxins on seed yield parameters in wheat, pea and canola grown under controlled environmental and Western Canadian field conditions. M. Sc. Thesis (Edmonton: University of Alberta.); 2015.

Jackson ML. Soil Chemical Analysis. Prentic-Hall, Indian; 1973.

Piper GS. Soil and Plant Analysis. Interscience Publishers, Inc. New York. 1947;368.

Bremner JM, Mulvaney CS. Total nitrogen. A. L., R. H. Miller and D. R. Keeny (Ed.). Methods of Soil Analysis. Part 2, Amer. Soc. Agron. Madison, W. I. USA. 1982;595-624.

Olsen SR, Sommers LE. Phosphorus. In: Page, R. H. Miller and D. R. Keeney (Ed.). Methods of Soil Analysis. Part 2 Amer. Soc. Agron. Madison, W. I. USA. 1982;403-430.

Horneck DA, Hanson D. Determination of potassium and sodium by Flam Emission Spectrophotometry. In Handbook of referance methods for plant analysis. Kalra YP. (Ed.). 1998; 153-155.

Kelly JD, Bliss FA. Heritability estimates of percentage seeds protein and available methionine and correlations with yield in dry bean. Crop Sci. 1975;15:753-757.

Dubois M, Gilles KM, Hamilton JK, Robers PA, Smith F. Calorimetric method for determination of sugars and related substances. Anolyt. Chem. 1975;28:350.

Bricker B. MSTATC: A micro computer program from the design management and analysis of agronomic research experiments. Michigan State Univ. USA; 1991.

Rouphael YL, Spíchal K, Panzarová R, Casa, Colla G. High-throughput plant phenotyping for developing novel biostimulants: From lab to field or from field to lab? Frontiers in Plant Science. 2018;9:1197.


Pace FB, Cantore V, Leo L, Vanadia S, Palma N, Phillips ED. Effect of particle film technology on temperature, yield and quality of processing tomato. Acta Horti. 2007;758:287–293.

Vashi JM, Saravaiya SN, Patel AI, BN Chaudhari. Silicon – The most under-appreciated element for vegetables. International J. of Chemical Studies. 2020;8(4):2122-2127.

Abdel-Aziz MA. Some physiological studies on tomato plant. Ph. D. Thesis, Fac. Agric., Cairo Univ., Egypt; 2007.

Kamal AM. Influence of irrigation levels, antitranspirants and potassium silicate on growth, fruit yield and quality of sweet pepper plants (Capsicum annuum L.) grown under drip irrigation. J. Plant Production, Mansoura Univ. 2013;11:1581 – 1597.

Abdel-Aziz MA, Geeth RHM. Effect of foliar spray with some silicon sources and paclobutrazol on growth, yield and fruit quality of sweet pepper (Capsicum Annuum L.) plants under high temperature conditions. Egypt. J. Agric. Res. 2018;2:577–593.

Abdel-Aziz MA, Geeth RHM. Effect of spraying by some substances on low temperature stress for growth and productivity in late peas (Pisum sativum L.) planting under the middle Egypt region conditions. J. Plant Production, Mansoura Univ. 2017;8:859 – 867.

El-Sawy SM, Fawzy ZF, El-Bassiony AM, Mahmoud SM. Effect of Nano bio-selenium and mineral selenium, ascorbic acid and salicylic acid on vegetative growth and fruit yield of pea plants grown under sandy soil conditions. International J. of Environment. 2019;4:2706-7939.

Waraich E, Ahmad R, Halim A, Aziz T. Alleviation of temperature stress by nutrient management in crop plants: a review. J. Soil Sci. Plant Nutr. 2012; 12:221–244.

DOI: 10.4014/jmb, 05009.

Gabriela M, Pastori GK, John A, Stephanie B, Sonja V, Noctor NG, Foyer F. Leaf vitamin C contents modulate plant defense transcripts and regulate genes that control development through hormone signaling. The Plant Cell. 2003;15: 939-951.

Munne BS. The role of α-tocopherols in plant stress tolerance. J. Plant Physiol. 2005;162:743- 748.

Shokr MMB, Abdelhamid MT. Using some antioxidant substances for enhancing thermotolerance and improving productivity of pea (Pisum sativum l) plants under local environment of early summer season. Agric Res. J. Suez Canal Univ. 2009;9(1):69-76.

Mona NS, Karima FA. Potassium silicate and amino acids improve growth, flowering and productivity of summer squash under high temperature conditions. American-Eurasian J. Agric. and Environ. Sci. 2019;19 (2):74-86.

Naz MUL, Sakeena TH, Ali HS. Riaz A, Muhammad AA. Potential of foliar application of micronutrients on growth, yield, quality and nutraceutical properties of pea (Pisum sativum L.); 2022.

Al-Said MA, Kamal AM. Effect of foliar spray with folic acid and some amino acids on flowering yield and quality of sweet pepper. J. Agric. Sci. Mansoura Univ. 2008;33(10):7403-7412.

Kadam MB, Jagtap PB, Chalak SU. Effect of foliar sprays of different nutrients on growth and yield of pea (cv. Arkel). Advances in Life Sci. 2016;5(20):9259-9263.

Muhsen HM, Jasim JA. Effect of boron, amino acids and silicon spraying on pea yield. Plant Archives. 2022;20(2): 3901-3904.

Pavlovic J, Kostic L, Bosnic P, Kirkby EA, Nikolic M. Interactions of silicon with essential and beneficial elements in plants. Front. Plant Sci. 2021;12. DOI:10.3389/fpls, 697592

Halpern M, Bar-Tal A, Ofek M. The use of biostimulants for enhancing nutrient uptake. In: Advances in Agronomy, Sparks, DL (Ed.), Academic Press, New York, NY, USA. 2015;141-174.

Chuntao T, Zhang L, Duan X, Chai X, Huang R, Kang Y, Yang X. Effects of exogenous sucrose and selenium on plant growth, quality and sugar metabolism of pea sprouts. J. Sci. Food Agric. 2022;102: 2855–2863.

Marcela Ž, Juríková T, Hegedűsová A, Golian M, Mlček J, Ryant P. The effect of selenium application on plant health ndicators of garden pea (Pisum sativum l.) varieties. Acta Univ. Agric. Silviculturae Mendelianae Brunensis. 2018;2:399–405.