Physiological Mechanism Involved in the Response to Four Lettuce Varieties to a Pre-transplant Root Restriction and a 6, benzyl aminopurine (BAP) Spray

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L. Campolongo
D. Carnelos
J. Lozano Miglioli
P. Fuginuma
E. Giardina
A. Di Benedetto


Vegetable yield is positively related to the environment and negatively affected by the pot root restriction during both the nursery and post-transplant stages. Root restriction is a physical stress imposed on the root system when plants are grown in small containers, which leads to a pronounced decrease in root and shoot growth at both the transplant and pot stages. Based on the assumption that the plant responses are mainly associated with a negative hormonal signaling from roots, some researchers have proposed that these abiotic stresses may be overridden by using a pre-transplant spray with benzyl amino purine (BAP), a synthetic cytokinin able to regulate plant metabolism. Although the physiological mechanisms induced by BAP have been described, the implementation of commercial applications of BAP for vegetables is still a pending issue. The aim of this work was to analyze growth changes in four lettuce genotypes in the presence of different root restrictions degree by the use of different plug cell volumes but sprayed with a single BAP spray under the hypothesis that it would play a role as abiotic stress alleviators. Our results showed that the higher biomass accumulation in lettuce plants non root-limited and BAP-sprayed ones are supported by higher photosynthetic rates, by higher leaf number initiation and expansion and by photo assimilate partition to shoots. Understanding the plant responses to this hormonal manipulation and the physiological mechanism involved will allow adjusting the agronomic advice for different vegetables and reaching commercial yields to each of them.

Cytokinin, growth parameters, nursery, transplant

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How to Cite
Campolongo, L., Carnelos, D., Miglioli, J. L., Fuginuma, P., Giardina, E., & Benedetto, A. D. (2020). Physiological Mechanism Involved in the Response to Four Lettuce Varieties to a Pre-transplant Root Restriction and a 6, benzyl aminopurine (BAP) Spray. Asian Journal of Agricultural and Horticultural Research, 5(4), 27-43.
Original Research Article


Di Benedetto A, Giardina E, De Lojo J, Gandolfo E, Hakim G. Exogenous benzyl amino purine (BAP) applications for the ornamental pot industry. In: Cytokinins: Biosynthesis and Uses (Ed. Sonja Kortesmäki), Nova Science Publishers, Inc. NY, USA. 2020;1-56.

Kudo T, Kiba T, Sakakibara H. Metabolism and long-distance translocation of cytokinins. J. Integr. Plant Biol. 2010;52: 53-60.

Kieber JJ, Schaller GE. Cytokinins. The Arabidopsis Book. 2014;12:e0168.

Di Benedetto A, Rattin J, Carnelos D, Lozano-Miglioli J, Giardina E, Araki A, Coro M, Pico-Estrada O, Teruel J, Di Matteo J, Gerasi J, Barrera L, Alonso E, Grigoli L. Technological uses of exogenous cytokinins in vegetables. In: Cytokinins: Biosynthesis and Uses (Ed. Sonja Kortesmäki), Nova Science Publishers, Inc. NY, USA. 2020;107-155.

Stagnari F, Galieni A, Pisante M. Shading and nitrogen management affect quality, safety and yield of greenhouse-grown leaf lettuce. Sci. Hortic-Amsterdam. 2015;192: 70-79.

Shehata SM, Schmidhalter U, Valšíková M, Junge H. Effect of biostimulants on yield and quality of head lettuce grown under two sources of nitrogen. Gesunde Pflanz. 2016;68:33-39.

Coro M, Araki A, Rattin J, Miravé P, Di Benedetto A. Lettuce and celery responses to both BAP and PBZ related to the plug cell volume. Amer. J. Exp. Agric. 2014;4: 1103-1119.

Di Benedetto A, Tognetti J. Técnicas de análisis de crecimiento de plantas: su aplicación a cultivos intensivos. RIA. 2016;42:258-282.

Warton DI, Duursma RA, Falster DS, Taskinen S. SMATR 3-an R package for estimation and inference about allometric lines. Methods Ecol. Evol. 2012;3:257-259.

Di Benedetto A. Root restriction and post-transplant effects for bedding pot plants. In: Aquino, J.C., Editor. Ornamental Plants: Types, Cultivation and Nutrition. New York, NY, USA. Nova Science Publishers, Inc. USA. 2011;47-79.

Araki A, Rattin J, Di Benedetto A, Miravé P. Temperature and cytokinin relationships on lettuce (Lactuca sativa L.) and celery (Apium graveolens L.) nursery growth and yield. Int. J. Agric. Res. 2007;2:725-730.

Salinas M, Gandolfo E, Hakim G, Giardina E, Di Benedetto A. Foliar amino acids sprays on lettuce (Lactuca sativa L.) biomass accumulation. Int. J. Curr. Microbiol. Appl. Sci. 2019;8:2183-2196.

Di Matteo J, Rattin J, Di Benedetto A. Increase of spinach growth through the use of larger plug cell volume and an exogenous BAP spray. Amer. J. Exp. Agric. 2015;6:372-383.

Geraci J, Di Matteo J, Feuring V, Giardina E, Di Benedetto A. Exogenous BAP spray on biomass accumulation in spinach. J. Exp. Agric. Int. 2018;25:1-17.

Della Gaspera P, Teruel J, Giardina E, Di Benedetto A. Physiological and technological consequences of benzyl adenine (BAP) application on Butternut squash (Cucurbita moschata Duchesne ex Poir.) productivity. Amer. J. Exp. Agric. 2016;13:1-11.

Rattin J, Pico Estrada O, Giardina E, Di Benedetto A. Nursery pre- and post-transplant effects on tomato (Solanum lycopersicum L.) growth and yield. J. Exp. Agric. Int. 2017;18:1-14.

Poorter H, Niklas KJ, Reich PB, Oleksyn J, Poot P, Mommer L. Biomass allocation to leaves, stems and roots: Meta‐analyses of interspecific variation and environmental control. New Phytol. 2012;193:30-50.

Arkhipova TN, Prinsen E, Veselov SU, Martinenko EV, Melentiev AI, Kudoyarova GR. Cytokinin producing bacteria enhance plant growth in drying soil. Plant Soil. 2007;292:305-315.

Shi K, Ding XT, Dong DK, Zhou YH, Yu JQ. Root restriction-induced limitation to photosynthesis in tomato (Lycopersicon esculentum Mill.) leaves. Sci. Hortic-Amsterdam. 2008;117:197-202.

Shi K, Fu LJ, Dong DK, Zhou YH, Yu JQ. Decreased energy synthesis is partially compensated by a switch to sucrose synthase pathway of sucrose degradation in restricted root of tomato plants. Plant Physiol. Bioch. 2008;46:1040-1044.

Carpenter S, Vertommen A, Swennen R, Witters E, Fortes C, Souza MT, Panis B. Sugar-mediated acclimation: The importance of sucrose metabolism in meristems. J. Proteome Res. 2010;9:5038-5046.