Biomass Accumulation in the Fern Asplenium nidus avis (L) under Root Restriction

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A. Pagani
J. Molinari
E. Giardina
A. Di Benedetto


Pot ornamental plant productivity is related to the environmental growth facilities but negatively affected by the pot root restriction syndrome. Most ferns showed a lower relative growth rate and long production cycles (24 months or more) for which growers use small pots to increase yield per unit area of greenhouse. The aim of this work was to analyze growth changes in response to different pot volume in plants of A. nidus avis spore-propagated under the hypothesis that it would play a role as an abiotic stress which decrease commercial productivity. Our results showed that the use of big pots increased fresh and dry weight and frond area (the main aesthetic trait). When growth parameters were performed, a higher the frond appearance rate (RLA), the frond area expansion (RLAE) and the frond thickness (SLA) were found in 1500 cm3 pot as well as the relative growth rate (RGR) and the net assimilation rate. The use of biggest pot for fern cropping stimulated biomass accumulation through a higher capacity to initiate and expand fronds, to increase photosynthetic rates and change photo assimilate partitioning which favor shoots. From the grower´s point of view, our results suggested that higher yields of A. nidus avis fern would be reached decreasing root restriction, that is, to use the biggest pot volume from the early transplant from plug trays.

Frond initiation, frond expansion, ornamental pteridophytes, photo assimilate partitioning

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How to Cite
Pagani, A., Molinari, J., Giardina, E., & Benedetto, A. D. (2020). Biomass Accumulation in the Fern Asplenium nidus avis (L) under Root Restriction. Asian Journal of Agricultural and Horticultural Research, 6(4), 1-9.
Original Research Article


Ranil RHG, Beneragama CK, Pushpakumara DKNG, Wijesundara DSA. Ornamental Pteridophytes: an underexploited opportunity for the Sri Lankan floriculture industry. J. Nat. Sci. Foundation Sri Lanka. 2015;43:293-301.

Brumfield RG, DeVincentis AJ, Wang X, Fernandez RT, Nambuthiri S, Geneve RL, Koeser AK, Bi G, Li T, Sun Y, Niu G, Cochran D, Fulcher A, Stewart JR. 2015. Economics of utilizing alternative containers in ornamental crop production systems. HortTechnol. 2015;25:17-25.

Page CN. Ecological strategies in fern evolution: a neopteridological overview. Rev. Palaeobot. Palynol. 2002;119:1-33.

Liao JX, Zhang H, Mo L, Huang YQ, Sun YJ, Li YQ. Differences in growth and biomass allocation of Adiantum flabellulatum and A. capillus-veneris as a result of light and water availability. Bot. Lett. 2017;164:393-400.

Di Benedetto A, Giardina E, De Lojo J, Gandolfo E, Hakim G. Exogenous Benzyl Amino Purine (BAP) Applications for the Ornamental Pot Industry. In: Cytokinins: Biosynthesis and Uses (Ed. Sonja Kortesmäki), Nova Science Publishers, Inc. NY, USA. 2020;1-56.

Di Benedetto A, Galmarini C, Tognetti J. New insight into how thigmomorphogenesis affects Epipremnum aureum L.: Interactions between vine training system and exogenous auxin and cytokinin action in plant development. Hortic. Bras. 2018;36:330-340.

Shi K, Ding XT, Dong DK, Zhou YH, Yu JQ. Root restriction-induced limitation to photosynthesis in tomato (Lycopersicon esculentum Mill.) leaves. Sci. Hortic-Amsterdam 2008;117:197-202.

Martin CE, Lin TC, Hsu CC, Lin SH, Lin KC, Hsia YJ, Chiou WL. Ecophysiology and plant size in a tropical epiphytic fern, Asplenium nidus, in Taiwan. Int. J. Plant Sci. 2004;165:65-72.

Di Benedetto A, Tognetti J. Técnicas de análisis de crecimiento de plantas: su aplicación a cultivos intensivos. RIA. 2016;42:258-282.

Ravi BX, Varuvel GVA, Robert J. Apogamous sporophyte development through spore reproduction of a South Asia's critically endangered fern: Pteris tripartita Sw. Asian Pacific J. Rep. 2015;4:135-139.

Cruz R, Melo de Pinna G F, Vasco A, Prado J, Ambrose BA. Class I KNOX Is Related to Determinacy during the Leaf Development of the Fern Mickelia scandens (Dryopteridaceae). Int. J. Molec. Sci. 2020;21:4295.

Vasco A, Ambrose BA. Simple and divided leaves in Ferns: Exploring the genetic basis for leaf morphology differences in the genus Elaphoglossum (Dryopteridaceae). Int. J. Molec. Sci. 2020;21:5180.

Lundgren MR, Fleming AJ. Cellular perspectives for improving mesophyll conductance. Plant J. 2020;101:845-857.

Ren T, Weraduwage SM, Sharkey TD. Prospects for enhancing leaf photosynthetic capacity by manipulating mesophyll cell morphology. J. Exp. Bot. 2019;70:1153-1165.

Di Benedetto A, Galmarini C, Tognetti J. Exogenous cytokinin promotes Epipremnum aureum L. growth through enhanced dry weight assimilation rather than through changes in partitioning. Amer. J. Exp. Agric. 2015;5:419-434.

Benedetto AD, Galmarini C, Tognetti J. Differential growth response of green and variegated Ficus benjamina to exogenous cytokinin and shade. Ornamental Hort. 2020;26:259-276.

Molinari J, Fujinuma P, Pagani A, Buyatti M, Giardina E, Di Benedetto A. Impatiens ‘New Guinea’ (Impatiens hawkeri Bull) propagation effects on the pre-transplant biomass accumulation. Int. J. Adv. Agric. Sci. 2018;9:1-1-15.

Molinari, J., Fujinuma P, Pagani A, Buyatti M, Giardina E, Di Benedetto A. Impatiens ‘New Guinea’ (Impatiens hawkeri Bull) hormonal effects during the post-transplant biomass accumulation. J. Adv. Agric. 2019;10:1629-1649.

O’Hare TJ, Turnbull CG. Root growth, cytokinin and shoot dormancy in lychee (Litchi chinensis Sonn.). Sci. Hort-Amsterdam. 2004;102:257-266.

Menéndez V, Abul Y, Bohanec B, Lafont F, Fernández H. The effect of exogenous and endogenous phytohormones on the in vitro development of gametophyte and sporophyte in Asplenium nidus L. Acta Physiol. Plantarum. 2011;33:2493-2500.

Di Benedetto A, Rattin J, Carnelos D, Lozano-Miglioli J, Giardina E, Araki A, Coro M, Pico-Estrada O, Teruel J, Di Matteo J, Gerasi J, Barrera L, Alonso E, Grigoli L. Technological Uses of Exogenous Cytokinins in Vegetables. In: Cytokinins: Biosynthesis and Uses (Ed. Sonja Kortesmäki), Nova Science Publishers, Inc. NY, USA. 2020;107-155.