Single Stem Rouging of Banana Xanthomonas Wilt Infected Plants for Orchard Rehabilitation
Asian Journal of Agricultural and Horticultural Research,
Banana Xanthomonas Wilt (BXW), caused by Xanthomonas campestris pv. musacearum (Xcm), a devastating disease, causes up to 100% loss and affects all banana varieties. The disease is widespread in East and Central Africa region. Study objective was to evaluate the performance of single stem rouging options for rehabilitation of severely affected banana orchard. Five single stem rouging options were implemented in banana orchard with 80% BXW severity in Western Kenya. The options included rouging of infected banana plants; uprooting, cutting at the base, injecting 20 ml of glyphosate herbicide at the soil base of the pseudostem, uprooting the whole stool and control leaving the infected stools. Initial visual assesment before implementation of the orchard was over 80% infection. Later samples were collected for diagnosis of ten plants per treatment from different plant parts and were subjected to ELISA and PCR procedures for confirmion of the presence of Xcm. Rouging options was implemented for one year. During and after experimentation periodic visual assesment of BXW incidence and severity reduced. Results of ELISA assays and PCR results indicated Xanthomonas was present on banana plants. Rouging the infected banana stems by cutting at the base, uprooting and injection of herbicide reduced BXW incidence within six months. Xanthomonas, an inhibiting bacteria cannot surve without host and by killing the banana plant the Xanthomonas dies. Yields were recovered from the rehabilitated orchard within one year. Banana orchard highly infected with BXW was effectively rehabilitated.
- Musa spp
- bacterial wilt
- ELISA Xanthomonas
How to Cite
Carter BA, Reeder R, Mgenzi SR, Kinyua ZM, Mbaka JN, Doyle K, Mwangi M, Valentine G, Aritua V, Ivey ML, Miller SA and Smith JJ. Confirmation of Xanthomonas vasicola, causative organism of Banana Xanthomonas Wilt, in Tanzania, Burundi and Kenya. Plant Pathology. 2009;57:170-177.
Tripathi L. Genetic transformation of bananas for resistance to Xanthomonas wilt disease. 2nd National Biosafety Conference 5-9 August, 2013, Nairobi Kenya. International Institute for Tropical Agriculture.
ProMusaBanana Xanthomonas wilt. 2014. BXW.www.ProMusa.org/Xanthomonas+wilt 7 April 2014.
Turyagyenda LF, Blomme G, Ssekiwoko F, Karamura E, Mpiira S and Eden-Green S. Rehabilitation of banana farms destroyed by Xanthomonas wilt in Uganda. Journal of Applied BioSciences. 2008;8:1:230– 235.
Mwangi Maina. Removing infected banana mats to contain Xanthomonas Wilt: Experiences in Uganda, Rwanda and the Democratic Republic of Congo. A brief prepared for the Crop Crisis Control Project. International institute of tropical agriculture Kampala, Uganda. 2007;1-13.
Mwangi M, Nakato V. Key factors responsible for the banana Xanthomonas wilt pandemic on banana in East and Central Africa. In: Recent advances in banana crop protection for sustainable production and improved livelihoods. ISHS ProMusa Symposium. Acta Hort. 2007; 828:395–404.
Di Cori V, Kikulwe E, Kozicka M, Gotor E. Understanding the economic impact of BXW and its management practices in East and Central Africa. Bioversity International, Rome, Italy. 2018;16.
Ndungo V, Eden-Green S, Blomme G, Crozier J, Smith J. Presence of banana Xanthomonas wilt (Xanthomonas campestris pv. musacearum) in the Democratic Republic of Congo. Plant Pathology. 2005;55:294.
Mwebaze JM. Studies on the survival of Xanthomonas campestris pv. musacearum in soil and plant debris, their importance as a source of inoculum for the next banana crop MSc. Thesis. Makerere University; 2006.
Karamura EB, Turyagyenda FL, Tinzaara W, Blomme G, Ssekiwoko F, Eden-Green S, Molina A and Markmham R. Xanthomonas wilt of banana in East and Central Africa. Diagnostic and management guide. Bioversity International, Uganda; 2008.
Kwach JK, Muthomi JW, Nderitu JH, Odongo M and Okoko N. Single stem rouging options for rehabilitation of Banana Xanthomonas Wilt affected plantations. 2016. 3rd International Interdisciplinary Conference, on 7 Sept 2016, University of Eldoret. Kenya.
Ocimati W, Ssekiwoko F, Karamura E, Tinzaara W, Eden-Green S and Blomme G. Systemicity of Xanthomonas campestris pv. musacearum and time to disease expression after inflorescence infection in East African highland and Pisang Awak bananas in Uganda. Plant Pathology. 2012;1-9.
Yirgou D and Bradbury F. Bacterial wilt of enset (Ensete ventricosa) incited by Xanthomonas pv musacearum. Phytopathology. 1968; 58: 111 - 112
Yirgou D and Brudbury F. A note on wilt of Banana caused by the Enset wilt organism, Xanthomonas musacearum. East Africa Agricultural and Forestry Journal. 1974;40:111-114.
Addis T, Turyagyenda LF, Alemu T, Karamura E and Blomme G. Garden Tool Transmission of Xanthomonas campestris pv. musacearum on Banana (Musa spp.) and Enset in Ethiopia Acta Hort. 2010; 879:367- 372.
Uwamahoro F, Berlin A, Bylund H, Bucagu C and Yuen J. Management strategies for banana Xanthomonas wilt in Rwanda include mixing indigenous and improved cultivars Agronomy for Sustainable Development. 2019;39:22.
Tinzaara W, Karamura EB, Kubiriba J, Ochola D, Ocimati W and Blomme G. Eds.: I. Van den Bergh et al. The banana Xanthomonas wilt epidemic in east and central Africa: current research and development efforts. Acta Hort. 2016; 1114. 36 XXIX IHC 267-274
Buregyeya H, Kubiriba J, Tusiime G, Kityo R, Ssekiwoko F and Tushemereirwe WK. Role of birds and bats in long distance transmission of banana bacterial wilt in Uganda. IJAIR. 2014;2(4):636–640.
Kubiriba J, Muthomi J, Ndungo V, Kwach J, Rockefeller E, Rwomushana I, Tushemereirwe W and Opio F. Strategies for rehabilitation of banana fields infested with Xanthomonas camptris pv musacearum. Journal of Crop Protection. 2014;3(1):21-29.
Kubiriba J and Tushemereirwe W. Approaches for the control of banana Xanthomonas wilt in East and Central Africa. African Journal of Plan Science. 2014;8(8):398-404.
Onyango M, Kwach J, Muthomi J. Management of Banana Xanthomonas wilt in Kenya. ASARECA and Kenya Agricultural Research Institute. Nairobi. Kenya; 2014.
Blomme G, Ocimati W, Sivirihauma C, Vutseme L, Mariamu B, Kamira M, van Schagen B, Ekboir J, Ntamwira JA control package revolving around the removal of single diseased banana stems is effective for the restoration of Xanthomonas wilt infected fields. European Journal of Plant Pathology. 2017;149(2):385–400.
Clark MF and Bar-Joseph. Enzyme-linked immunosorbent assay in plant virology. In: Marmorash K, Korowski H, editors. Methods in virology. New York: Academic Press. 1984;51–85.
Hobbs HA, Reddy DVR, Rajeshwari R and Reddy SA. Use of direct antigen coating and protein A coating ELISA procedures for three peanut viruses. Plant Disease. 1987;71:747-749.
Cerovska C, Filigarova´ NM and Pecˇenkova´ T. Production of polyclonal antibodies to a recombinant potato mop-top virus non- structural triple gene block protein 1. Journal Phytopathology. 2006; 154:422–427.
Ssekiwoko F, Taligoola HK and Tushemereirwe WK. Xanthomonas campestris p.v. musacearum. Host range in Uganda. African Crop Science Journal. 2006;14(2):111-120.
Vemana K, Jain RK. New Experimental Hosts of Tobacco streak virus and Absence of True Seed Transmission in Leguminous Host. Indian Journal of Virology. 2010;21(2):117–127.
Studholme DJ, Kemen E, MacLean D, Schornack S, Aritua V. Genome-wide sequencing data reveals virulence factors implicated in BXW. Federation of European Microbiological Societies. Microbiology Letters. 2010;310(2):182–192.
Ngobich H, Albrechtsen SE, Nicolaisen M. Detection and identification of the blackeye cowpea mosaic strain of bean common mosaic virus in seeds of Vigna unguiculata sp. from North Vietnam. Australasian Plant Pathology. 2003;32:505–509.
Rajasulochana P, Dhamotharan R, Srinivasulu P. Comparison of Dac-Elisa and Dot-Blot-Elisa for the detection of cucumber mosaic and banana streak viruses infecting banana. The Journal of American Science. 2008;4(2):49.
Kwach KJ. Occurrence of Banana Xanthomonas Wilt in Kenya and potential approaches to rehabilitation of infected orchards Ph.D. Thesis in Crop Protection. University of Nairobi, Kenya; 2014.
McCampbell M, Schut M, Van den Bergh I, Van Schagen B. Xanthomonas wilt of banana in Central Africa: opportunities, challenges, and pathways for citizen science and ICT-based control and prevention strategies. Journal of Life Science. 2018;86(87):89–100.
Kikulwe EM, Okurut S, Ajambo S, Gotor E, Ssali T, Kubiriba J, Karamura E. Does gender matter in effective management of Banana Xanthomonas Wilt? Insights from a survey among rural banana farming households in Uganda. Journal of Development and Agricultural Economics. 2018;10(3):87-98.
Okurut WA, Tushemereirwe WK, Aritua V and Ragama PE. Use of herbicide for control of banana bacterial wilt in Uganda. African. Crop Science Journal. 2006;14(2): 143-14936.
Kitavi M, Downing T, Lorenzen J, Karamura D, Onyango M, Nyine M, Ferguson M, Spillane C. The triploid East African Highland Banana (EAHB) genepool is genetically uniform arising from a single ancestral clone that underwent population expansion by vegetative propagation. Theoretical and Applied Genetics. 2016; 129:547–561.
Ndayitegeye O, Onyando OJ, Okwany OR, Kwach KJ. Vegetative growth of banana as inﬂuenced by deﬁcit irrigation and irrigation interval. Fundamental and Applied Agriculture. 2019;4(4):1047–1053.
Onyango MA, Kwach JK, Okoko N. Banana Production Field Technical Guide. Africa Harvest Boitec Foundation International, Nairobi. Kenya; 2015.
Ssekiwoko F, Taligoola HK and Tushemereirwe WK. Xanthomonas campestris p.v. musacearum. Host range in Uganda. African Crop Science Journal. 2006;4(2):111–120.
Ramathani I, Beed F. Use of DNA capture kits to collect Xanthomonas campestris p.v. musacearum and banana bunchy top virus pathogen DNA for molecular diagnostics, In. Banana systems in the humid highlands of sub-Saharan Africa: enhancing resilience and productivity (Eds) Blomme, G.; Asten, P. van; B. Vanlauwe. CAB International Printed and bound in the UK by CPI Group (UK) Ltd, Croydon, CR0 4YY. 2013;109-115.
Smith JJ, Jones DR, Karamura E, Blomme G and Turyaenda L. An analysis of risk from Xanthomonas campestris p.v. musacearum to banana cultivation in East, Central and Southern Africa. Bioversity International, Montepellier, France; 2008.
Welde-Michael G, Bobosha K, Addis T, Blomme G, Mekonnen S and Mengesha T. Mechanical transmission and survival of bacterial wilt (Xanthomonas campestris p.v. musacearum) on enset (Ensete ventricosum). African Crop Science Journal. 2008;16(1):89-95.
Tumushabe A, Ssekiwoko F and Tushemereirwe WK. Potential of infected banana parts to transmit Xanthomonas campestris p.v. musacearum. African Crop Science Journal. 2006;14:137-142.
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