Single Stem Rouging of Banana Xanthomonas Wilt Infected Plants for Orchard Rehabilitation

Johnson Kisera Kwach *

Faculty of Biological and Physical Sciences, Tom Mboya University College, P.O.Box 199-40300, Homa Bay, Kenya.

Peter Sungu Nyakomitta

Faculty of Biological and Physical Sciences, Tom Mboya University College, P.O.Box 199-40300, Homa Bay, Kenya.

James Wanjohi Muthomi

epartment of Plant Science and Crop Protection, University of Nairobi, P.O.Box 30197, Nairobi Kenya.

Olivier Ndayitegeye

Rwanda Agriculture and Animal Resources Development Board, Private Bag, Kigali, Rwanda.

Evelyn Nasambu Okoko

Kenya Agricultural and Livestock Research Organization, P.O.Box 523-40200, Kisii, Kenya.

*Author to whom correspondence should be addressed.


Abstract

Banana Xanthomonas Wilt (BXW), caused by Xanthomonas campestris pv. musacearum (Xcm), a devastating disease, causes up to 100% loss and affects all banana varieties. The disease is widespread in East and Central Africa region. Study objective was to evaluate the performance of single stem rouging options for rehabilitation of severely affected banana orchard. Five single stem rouging options were implemented in banana orchard with 80% BXW severity in Western Kenya. The options included rouging of infected banana plants; uprooting, cutting at the base, injecting 20 ml of glyphosate herbicide at the soil base of the pseudostem, uprooting the whole stool and control leaving the infected stools. Initial visual assesment before implementation of the orchard was over 80% infection. Later samples were collected for diagnosis of ten plants per treatment from different plant parts and were subjected to ELISA and PCR procedures for confirmion of the presence of Xcm. Rouging options was implemented for one year. During and after experimentation periodic visual assesment of BXW incidence and severity reduced. Results of ELISA assays and PCR results  indicated Xanthomonas was present on banana plants. Rouging the infected banana stems by cutting at the base, uprooting and injection of herbicide reduced BXW incidence within six months. Xanthomonas, an inhibiting bacteria cannot surve without host and by killing the banana plant the Xanthomonas dies. Yields were recovered from the rehabilitated orchard within one year. Banana orchard highly infected with BXW was effectively rehabilitated.

Keywords: Musa spp, bacterial wilt, replanting, glyphosate, removing, PCR, ELISA Xanthomonas


How to Cite

Kisera Kwach, Johnson, Peter Sungu Nyakomitta, James Wanjohi Muthomi, Olivier Ndayitegeye, and Evelyn Nasambu Okoko. 2021. “Single Stem Rouging of Banana Xanthomonas Wilt Infected Plants for Orchard Rehabilitation”. Asian Journal of Agricultural and Horticultural Research 8 (4):12-25. https://doi.org/10.9734/ajahr/2021/v8i430122.

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