Metabolic Profiling of Healthy and Pest Infested Solanum melongena L. Using a Gas Chromatography-mass Spectroscopy Technique
Hema Bisht *
Department of Chemistry, Pt. Shambhu Nath Shukla Government P.G. College, Shahdol, MP-484001, India.
M. K. Bhatnagar
Department of Chemistry, Pt. Shambhu Nath Shukla Government P.G. College, Shahdol, MP-484001, India.
*Author to whom correspondence should be addressed.
Abstract
A comparative metabolic profiling of healthy and pest infested Solanum melongena (egg plant) have been carried out. The egg plant was infested by Helicoverpa armigera. Total thirty non polar metabolites were detected from the leaf and stems extract, by gas chromatography-mass spectroscopy (GC-MS) technique, which includes large variety of compounds like phytosterols, diterpene, alkane hydrocabon, n-alkanoic acid and terpene etc. Significant variation on metabolites has been detected in both leaf and stem extract. Metabolites such as benzoic acid (9.13±0.71%), pentadecane (2.83±0.13%), hexadecane (1.68±0.09%), squalene (1.28±0.08%), triterpene (3.97±0.14%), linoleic acid (1.32±0.06%), linolenic acid (2.46±0.14%), stearic acid (4.69±0.38%), dodecanoic acid (0.83±0.10%), myristic acid (1.25±0.05%), palmitic acid (1.32±0.06%), linoleic acid (3.33± 0.13%), linolenic acid (2.06±0.14%), stearic acid (4.83±0.14%), lactic acid (0.50±0.09%), tetradecene (0.93±0.07%), hexadecane (1.30±0.06%), octadecane (1.19±0.05%), cholesterol (0.48 ±0.05%), stigmasterol (0.57±0.06%), (octadecene 6.90±1.58%) and tetradecane (0.84±0.10%) were detected. Alteration in amount of above major metabolites was observed under biotic stress condition. It concludes that, these metabolites might have played an important role in pest infested stress tolerance. This study will be helpful for the better understanding of overall biotic stress tolerance mechanism.
Keywords: Solanum melongena, H. armigera, metabolites, pest infestation, gas chromatography-mass spectrometry